1) Shut down your vm
2) download your vm's vmx file (it's located in your vm directory). [follow these pictures, if you don't know how to download http://www.thomas-krenn.com/de/wiki..._zum_Kopieren_von_Dateien_auf_ESX/ESXi_Server]
3) insert a new line into your vmx file: as Benoire already stated
5) power on vm.
2) download your vm's vmx file (it's located in your vm directory). [follow these pictures, if you don't know how to download http://www.thomas-krenn.com/de/wiki..._zum_Kopieren_von_Dateien_auf_ESX/ESXi_Server]
3) insert a new line into your vmx file: as Benoire already stated
4) reupload your vmx file to the same location and overwrite the existing vmx file.So to add this item (and it is one per device passthrough and it must match the passthrough device number, i.e. 4 devices will yeild a final line of pcipassthru3.msienabled = false)
5) power on vm.